Today lonza introduced the 4dnucleofector tm lv unit to its transfection platforms. By continuing to browse the site, you are agreeing to our use of cookies. For preparing aliquots, mix nucleofector solution and supplement in a ratio of 4. The cell line nucleofectortmkit t is for transfection of cell lines in the nucleofectortm2b. None of the equipment posted for sale on is owned by medwow, should you have any questions regarding a.
However, if no cell typespecific protocol is available for your cell type of interest, transfection optimization is a simple, straightforward process. We have a amaxa nucleofector lonza in the lab and it seems that this is the best way to get dna in those cells. The 384well nucleofector tm system is an independent platform for highthroughput nucleofection in a 384well format. An independent nucleofectortm platform for highthroughput transfection in 384 well format. In addition, our knowledge database contains transfection data. Product overview the 4dnucleofector tm core unit is the controlling unit of the 4dnucleofector tm system. Lonza launches 4dnucleofector lv unit for largescale. Lonza nucleofector technology at tri translational research. Lonza walkersville cell line optimization 4d nucleofector x kit, 64 reactions manufacturer. Lonza nucleofector technology at tri translational. Date hs code description origin country port of discharge unit quantity value inr per unit inr oct 06 2016.
The nucleofector device delivers unique electrical parameters. Lonza walkersville v4xp3024 the p3 primary cell 4dnucleofector x kit l is one of five. Ingenio cuvettes for the ezporator electroporation system and lonzaamaxa nucleofector ii2b devices ingenio cuvettes for the ezporator electroporation system and all conventional electroporators such as biorad and harvardbtx ingenio cell droppers. Lonza walkersville p3 primary cell 4dnucleofector x kit l, 24 reactions, 100ul nucleocuvette vessels manufacturer. Lonzas 4dnucleofector lv unit delivers closed, optimized. Lonza provides readytouse, cell typespecific optimized protocols for more than 150 cell types. Purchasers are granted a nonexclusive, nontransferable license for a limited use of lonzas nucleofector technology and related software. The nucleofector tm 2b device is a successor of the original platform that was introduced in 2001 and enables lowthroughput transfection in a single cuvette format. They are based on cell culture methods, where live cells are grown in vitro and used as model systems to assess the biochemistry and physiology of both healthy and diseased cells. Lonza p3 primary cell 4dnucleofectortm x kit l contact us. Our solution is an improved electroporation technology, the nucleofector tm technology, originally introduced into the market by legacy amaxa in 2001.
Lonza walkersville v4xc9064 the cell line optimization 4d nucleofector x kit provides convenient and rapid determination of optimal nucleofection conditions for a broad range of primary cells within one experiment. The different functional units can be added in a modular way to the 4d nucleofector tm core unit and each is suited for different applications. Removal will begin on july 25th by scheduled appointment only. Aug 10, 2015 lonzas nucleofector technology is an improved electroporation technology that can help you achieve transfection results never before possible in primary cells, stem cells and hard to. Lonza walkersville v4xp3024 the p3 primary cell 4dnucleofector x kit l is one of five 4dnucleofector kits suited for transfecting primary cells in the 100 l nucleocuvettetm vessel format when working with the 4dnucleofectortm. Cellbased assays and analysis are vital experimental tools in life science research and biomanufacturing. Logos for editorial use are suitable to illustrate news articles, but are not cleared for. Unlike classical electroporation, nucleofection delivers high efficiencies that can be achieved using much lower amounts of substrate with only a moderate impact on. Lonza 4d nucleofector core unit w x and y unit nucleofector. If you have similar experiences, please feel free to contact us so we can elaborate a common database for the transfection of different cell lines. They are not compatible with the nucleofector ii2b device. Use of lonzas nucleofector technology andor related software requires a license from lonza cologne gmbh. The company was founded in 1998 and is based in cologne, germany. However, the culture looks like a graveyard 24h post transfection.
Pulse code used for hek293t cells and pbshepes buffer should be set to ff120. For all the electroporations using in house buffers, generic. Jun 07, 2016 lonzas nucleofector technology is an improved electroporation technique that has transformed transfection and allowed scientists to achieve results never before possible. All content is posted anonymously by employees working at lonza. The nucleofector technology relies on the combination of a nucleofector device and cell specifi c nucleofector kits. The controlling unit of the 4dnucleofectortm system is suited to handle various fuctional units for efficient transfection of hardtotransfect cell lines and primary. It provides value chemical and biotech ingredients to the nutrition and agro markets.
May 15, 2019 lonza group ag engages in the supply of pharmaceutical, healthcare, and life science products. Use of lonzas nucleofector technology andor related software. The company provides product development services to the pharmaceutical and biologic industries. Lonza was established under that name in the late 19thcentury in switzerland. Turn on all nucleofector hardware followed by nucleofector shuttle software. The translational research institute is a medical research facility bringing together the university of queensland, metro south health, queensland institute of technology and mater medical research to. Lonza 4dnucleofector core unit, sn 510b0359, with x unit. Logos for editorial use are suitable to illustrate news articles, but are not cleared for commercial use.
The 21 cfr part 11 compliant software for the 4dnucleofectortm system. Lonza cologne gmbh is holder of various patents, patent applications, s and technical and scientific experience with respect to the nucleofector technology. It enables highly efficient, transfection of primary cells, stem cells, neurons, and cell lines that have traditionally been difficult to transfect via electroporation and other nonviral. Recommended kitsp4 primary cell 4dnucleofector x kit amaxa 4dnucleofector protocol for mouse embryonic fibroblasts mef for 4dnucleofector x unit transfection in suspension validated to work with primary nonimmortalized mouse embryonic fibroblasts from lonza cat. Lonzas nucleofector technology is an improved electroporation technology that can help you achieve transfection results never before possible in primary cells, stem cells and hard to. Lonza 4dnucleofector core unit, sn 510b0359, with x unit optimized electroporation unit, sn 510x0429.
Cell and transfection, citation, faq, culture media or use the search below. Lonza group is a swiss multinational, chemicals and biotechnology company, headquartered in basel, with major facilities in europe, north america and south asia. The electrical settings are preprogrammed for each optimized cell type and can be selected via the device or pc software. Handmade solutions for amaxa lonza nucleofector facebook. It is a powerful tool for transfecting large dna fragments and achieving good transfection efficiencies in cell lines. Purchasers are granted a nonexclusive, nontransferable license for a limited use of amaxas nucleofector technology and related software. The different functional units can be added in a modular way to the 4dnucleofector tm core.
Nucleofector tm 2b device the basic, single cuvette based nucleofector tm system for efficient transfection of hardtotransfect cell lines and primary cells with different substrates e. View detailed import data, price, monthly trends, major importing countries, major ports of lonza. Product overview the 4d nucleofector tm core unit is the controlling unit of the 4d nucleofector tm system. The controlling unit of the 4dnucleofectortm system is suited to handle various. In addition, our knowledge database contains transfection data for close to 1500 cell types. Below, you will find the composition of the buffers and the supplement solutions. Nucleofection and dual luciferase detailed protocols.
Amaxa biosystems offers its solutions in canada, china, croatia, greece, hong kong, indonesia, japan, korea, malaysia, mexico, portugal, singapore, spain, taiwan, and turkey. Manufacturer specifications amaxa 4dnucleofector system, lonza please note. Trouble with amaxa nucleofection in my lab we need to deliver dna to b cells. Intuitive operation software for designing and saving individual experimental setups. The vision section contains a free online catalogue illustrating the use of organizational vision statements in practice by organizations from around the world. The nucleofector technology is a highly efficient nonviral method for transfection.
Today lonza introduced the 4dnucleofector tm lv unit to its transfection platforms the new unit expands the proven 4dnucleofector system to closed, largerscale transfection of up to. As of july 2, 2008, amaxa biosystems gmbh operates as a subsidiary of lonza group ag. If you have similar experiences, please feel free to contact us so we can elaborate a common database. None of the equipment posted for sale on is owned by medwow, should you have any questions regarding a specific item, please direct them to the appropriate seller by making use of the available communication channels on the items page. The translational research institute is a medical research facility bringing together the university of queensland, metro south health, queensland institute of technology and mater medical research to translate research findings into improved healthcare and increased income and jobs for australia. Note 4dnucleofector solutions can only be used with nucleocuvettes conductive polymer cuvettes, i. Jul 19, 2016 muellerhartmann went on to say, the fully closed system is also suited for use in a regulatory environment as it can be controlled by 21 cfr part 11compatible software.
The nucleofector device from lonza group ltd selectscience. The nucleofector tm technology uses a specific combination of optimized electrical parameters and cell typespecific solutions which enables transfer of a molecule directly into the cells nucleus. Manufacturer specifications amaxa 4dnucleofector system, lonza. For this system, we also offer alternative cuvettes that can be used for bacteria transformation. The mediumthroughput addon for the 4dnucleofectortm system suited for transfection. In difference to the above mentioned platforms that work with conductive polymer vessels, the nucleofector tm 2b device uses aluminium cuvettes. Order lonzas p3 primary cell 4dnucleofectortm x kit l on. Purchasers are granted a nonexclusive, nontransferable license for a limited use of lonzas nucleofector technology and related software for research and development purposes, the terms of which are disclosed in detail in chapter 5.
Lonza walkersville cell line optimization 4dnucleofector x. Lonza walkersville p3 primary cell 4dnucleofector x kit l. Cell culture assays provide a means of quantitatively analyzing the presence, amount, or functional activity. Logos database editorial logos find lonza logo image and details.
Use of lonza s nucleofector technology andor related software requires a license from lonza cologne gmbh. We have tried buffers to nucleofect different cell lines using the amaxa device. Efficient genome editing using nucleofector tm technology introduction to genome editing there are various options to modulate gene expression, be it on the dna, rna, or protein level. Lonza walkersville cell line optimization 4dnucleofector. Lonza amaxa 4dnucleofector system electronic equipment. Lonza cologne ag 50829 cologne, germany please note that the amaxa nucleofector technology is not intended to be used for diagnostic purposes or for testing or treatment in humans the nucleofector technology, comprising nucleofection process, nucleofector device, nucleofector solutions, nucleofector. The 384well nucleofector tm kits use existing 96well shuttle tm protocols with conductive polymer 384well nucleocuvette tm plates. Supplement is added to the nucleofector solution, it is stable for three months at 4 c. Amaxa 4dnucleofector optimization protocol for cell lines.
The core unit controlling the 4dnucleofector system. An efficient low cost method for gene transfer to t. The new unit expands the proven 4dnucleofector system to closed, largerscale transfection of up to. Lonza walkersville cell line optimization 4dnucleofector x kit, 64 reactions manufacturer. Yearly guarantee extension for the nucleofector tm 2b device that can be purchased together with the device or as long as the device is covered by an existing guarantee.
Protocols or recommendations available in our knowledge database. Lonza has launched its new 4dnucleofector lv unit, a new system designed to deliver largerscale transfection capabilities than its previous offerings. Lonza group ag engages in the supply of pharmaceutical, healthcare, and life science products. Mar 26, 20 the buffers used were provided from lonza human t cells nucleofector kit for human t cells cat number vpa1002, mouse t cell nucleofector kit for mouse t cells cat number vpa1006, or in house prepared buffer solutions for human and mouse lymphocytes described in table s1. They are resistant to easy transfection methods and lately we have been having issues with our outdated retroviral system. Recommended kitsp4 primary cell 4dnucleofector x kit amaxa 4dnucleofector protocol for mouse embryonic fibroblasts mef for 4dnucleofector x unit transfection in suspension. The controlling unit of the 4dnucleofector tm system is suited to handle various fuctional units for efficient transfection of hardtotransfect cell lines and primary cells in different formats. Nucleofector technology stretching transfection dimensions. With an extremely fast plate processing time of 1 minute per plate, it is perfectly suited for screening applications with maximum reproducibility. The 384well nucleofector tm system consists of a power supply unit generating the high voltage pulses, a plate handler unit and an intuitive pcbased operation software. Manufacturer specifications amaxa 4d nucleofector system, lonza please note. Purchasers are granted a nonexclusive, nontransferable license for a limited use of amaxas nucleofector technology and related software for research and development purposes, the terms of which are disclosed in detail in the license.
Lonza walkersville v4xc9064 the cell line optimization 4dnucleofector x kit provides convenient and. Lonzas nucleofector technology is an improved electroporation technique that has transformed transfection and allowed scientists to achieve results never before. Purchasers are granted a nonexclusive, nontransferable. Lonzas nucleofector technology is an improved electroporation technology that can help you achieve transfection results. To determine if an optimized protocol is available for your cell type of interest, search our knowledge database or contact the scientific support team. Use of lonzas nucleofector technology andor related software requires a license from lonza.